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SerolyzeR - an R package for automated analysis of serological data

Overview

This package is a simple tool that handles various raw data formats produced in Multiplex Bead Assay (MBA). In short, it reads the unstructured, raw data from, e.g., the Luminex device and outputs normalised and well-structured data, which can be used later in more advanced downstream analysis.

The package includes three main steps for preprocessing the data:

  1. data reading and manipulation
  2. quality control
  3. data normalisation

The graphical overview of the package can be seen in the image below:

overview

SerolyzeR package is developed within the PvSTATEM initiative. The project has received funding from the European Union’s Horizon Europe research and innovation program under grant agreement No 101057665.

Previously, this package was named PvSTATEM, but it has been rebranded to SerolyzeR to better reflect its purpose and scope.

Installation

The easiest way to install the package is using the CRAN repository:

install.packages("SerolyzeR")
require(SerolyzeR) # load the installed package

Now, you are ready to use the package to read your files!

Please note that since uploading the package to the CRAN repository requires the volunteers’ time to manually run checks on the packages, the package version currently released on CRAN might not be the latest.

The package is under heavy development, with new features being released weekly. Therefore, if you’d like to test the latest package functionalities, we recommend installing it in the development version. It can be done using a simple command install_github available in the devtools library:

require(devtools)
install_github("mini-pw/SerolyzeR")
require(SerolyzeR) # load the installed package

The first command loads the devtools library (you might need to install it first - using the command install_packages("devtools")), and the second one sources the git repository with the code of our package and automatically installs it.

Examples and instructions

The example use of the package and its functionalities can be found in the vignettes. For more detailed documentation, check the package website.

Contributing and issues

As a project in the development phase, we are open to any suggestions, bug reports, and contributions. If you have any ideas or issues, please report them in the Issues section. Our team of developers will address them as soon as possible.

Acknowledgements

Funded by the European Union. Views and opinions expressed are, however, those of the author(s) only and do not necessarily reflect those of the European Union or HaDEA. Neither the European Union nor the granting authority can be held responsible for them.

The package was developed as a Bachelor’s thesis by Jakub Grzywaczewski, Tymoteusz Kwieciński and Mateusz Nizwantowski on Warsaw University of Technology, Faculty of Mathematics and Information Science, supervised by prof. dr hab. inż. Przemysław Biecek.